Definition for Difference between revisions of "Sodium dodecyl sulfate"
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(Created page with "SDS (sodium dodecyl sulfate) is a detergent (soap) that can dissolve hydrophobic molecules but also has a negative charge (sulfATE) attached to it. Therefore, if a cell is incuba...") |
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| − | SDS (sodium dodecyl sulfate) is a detergent (soap) that can dissolve hydrophobic molecules but also has a negative charge (sulfATE) attached to it. Therefore, if a cell is incubated with SDS, the membranes will be dissolved and the proteins will be | + | SDS (sodium dodecyl sulfate) is a detergent (soap) that can dissolve hydrophobic molecules but also has a negative charge (sulfATE) attached to it. Therefore, if a cell is incubated with SDS, the membranes will be dissolved and the proteins will be solubilized by the detergent, plus all the proteins will be covered with many negative charges. The end result has two important features: |
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| + | 1) All proteins contain only primary structure and | ||
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| + | 2) All proteins have a large negative charge which means they will all migrate towards the positive pole when placed in an electric field. | ||
Latest revision as of 19:35, 8 November 2011
SDS (sodium dodecyl sulfate) is a detergent (soap) that can dissolve hydrophobic molecules but also has a negative charge (sulfATE) attached to it. Therefore, if a cell is incubated with SDS, the membranes will be dissolved and the proteins will be solubilized by the detergent, plus all the proteins will be covered with many negative charges. The end result has two important features:
1) All proteins contain only primary structure and
2) All proteins have a large negative charge which means they will all migrate towards the positive pole when placed in an electric field.