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Mastering Genetics: Transcription and RNA Processing
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For a more comfortable homework help experience, try HomeworkClinic.com.
Transcription and RNA Processing
During transcription, RNA polymerase synthesizes RNA from a DNA template with the help of accessory proteins. In this tutorial, you will review the steps of transcription in eukaryotes and bacteria and investigate splicing of mRNAs in eukaryotes.
Part A - Transcription in bacteria
The diagram below shows a length of DNA containing a bacterial gene.
Drag the labels to their appropriate locations in the diagram to describe the function or characteristics of each part of the gene. Not all labels will be used.

You labeled 3 of 5 targets incorrectly. Keep in mind that the origin of replication is involved in the copying of DNA, which is a different process than the synthesis of RNA from a DNA template.



Part B - Transcription in eukaryotes
Drag the labels into the flowchart to show the order of events as they are thought to occur during eukaryotic transcription involving RNA polymerase II (RNA pol II).

Clueless

Part C - Pre-mRNA splicing in eukaryotes
The following eukaryotic structural gene contains two introns and three exons.
Eukaryotic structural gene with two introns and three exons
The table below shows four possible mRNA products of this gene. Use the labels to explain what may have caused each mRNA.
Drag the correct label to each location in the table. Labels may be used once, more than once, or not at all.

Attempted once, but still have a missing answer.


Extra informationChapter 9; The Molecular Biology of Translation
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Mastering Genetics: Transcription and RNA Processing
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Is this Genetic Analysis: An Integrated Approach ?


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Mastering Genetics: Transcription and RNA Processing
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Part C - Pre-mRNA splicing in eukaryotes
The following eukaryotic structural gene contains two introns and three exons.
Eukaryotic structural gene with two introns and three exons
The table below shows four possible mRNA products of this gene. Use the labels to explain what may have caused each mRNA.
Drag the correct label to each location in the table. Labels may be used once, more than once, or not at all.

Attempted once, but still have a missing answer.

1) A. mutations in splicing signal sequences in intron 2. Intron 2 is still included in the mature mRNA, but it should have been spliced out. For this to happen, there would have been some kind of mutation in the splicing signal for that intron.

2) D. mutation in the gene's promoter sequence. No mRNA was produced, so transcription didn't even occur. Because the promoter sequence is required to initiate transcription, a mutation would need to occur in it for no mRNA to be produced.

3) B. mutation in splicing signal sequences in both intron 1 and intron 2. Because both intron 1 and intron 2 were in the mature mRNA and not spliced out like they should have been, there was a problem with the splicing signal sequences for both introns.

4) C. no mutation in any splicing signal sequences. All of the introns were spliced out of the mature mRNA, so the proper mRNA was produced. Thus, there were no mutations in any of the splicing signal sequences.


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Mastering Genetics: Transcription and RNA Processing
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Is this Genetic Analysis: An Integrated Approach ?

Yes


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Mastering Genetics: Transcription and RNA Processing
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Order of transcription factor binding:

TFIID binds to the TATA box
TFIIB binds to TFIID
TFIIF and RNA polymerase II binds to TFIIB and TFIID
TFIIH binds completing the initiation complex


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1. TFIIE and TFIIH bind

2. Synthesis of the pre-mRNA begins at the +1 nucleotides

3. Spliceosome complexes carry out intron splicing

4. TFIID binds to the TATA box

5. A 5' cap is added to the pre-mRNA

6. TFIIB, TFIIF, and RNA pol 2 bind

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4,6,2,1,5,3


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Mastering Genetics: Transcription and RNA Processing
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1. TFIIE and TFIIH bind

2. Synthesis of the pre-mRNA begins at the +1 nucleotides

3. Spliceosome complexes carry out intron splicing

4. TFIID binds to the TATA box

5. A 5' cap is added to the pre-mRNA

6. TFIIB, TFIIF, and RNA pol 2 bind

Quote
4,6,2,1,5,3

I figured it out actually. Your answer is slightly off, its: 4,6,1,2,5,3. (Switch 2 &1). But thanks for the input.

Anyone get the answer to 1 yet? I've looked in the book and still can't put together the answer. I already used up 1 of my 2 chances and want to be sure. 


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Mastering Genetics: Transcription and RNA Processing
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Thanks


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Got #1 right after the second try for anyone who needs it in the future



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Mastering Genetics: Transcription and RNA Processing
A year ago
Thanks for posting this guys, it was really helpful, because I was stuck on this problem


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Mastering Genetics: Transcription and RNA Processing
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Thanks! I was stuck on this!


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A year ago
I agree


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I agree also


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Thank you for posting this, I was having a very hard time figuring it out even with the hints.


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